JUHA ROUVINEN

Contact info

Keywords

Research groups - UEF

• 
  Bioorganic chemistry

Publications

• Chruszcz, Maksymilian; Chew, Fook Tim; Hoffmann-Sommergruber, Karin; Hurlburt, Barry K; Mueller, Geoffrey A; Pomes, Anna; Rouvinen, Juha; Villalba, Mayte; Woehrl, Birgitta M; Breiteneder, Heimo. 2021. Allergens and their associated small molecule ligands - their dual role in sensitization Allergy 76 8: 2367-2382. 2021
• Rouvinen, Juha; Andberg, Martina; Pääkkönen, Johan; Hakulinen, Nina; Koivula, Anu. 2021. Current state of and need for enzyme engineering of 2-deoxy-D-ribose 5-phosphate aldolases and its impact Applied microbiology and biotechnology 105 16-17: 6215-6228. 2021
• Tu, Tao; Wang, Zhiyun; Luo, Yan; Li, Yeqing; Su, Xiaoyun; Wang, Yuan; Zhang, Jie; Rouvinen, Juha; Yao, Bin; Hakulinen, Nina; Luo, Huiying. 2021. Structural Insights into the Mechanisms Underlying the Kinetic Stability of GH28 Endo-Polygalacturonase Journal of agricultural and food chemistry 69 2: 815-823. 2021
• Delfi, Masoud; Mahdavian, Leila; Sattarifar, Mohammad; Hakulinen, Nina; Rouvinen, Juha. 2021. Crystallization of 3-hexulose-6-phosphate synthase European journal of chemistry 12 3: 299-303. 2021
• Pääkkönen, Johan; Penttinen, Leena; Andberg, Martina; Koivula, Anu; Hakulinen, Nina; Rouvinen, Juha; Jänis, Janne. 2021. Xylonolactonase from Caulobacter crescentus Is a Mononuclear Nonheme Iron Hydrolase Biochemistry 60 41: 3046-3049. 2021
• Pääkkönen, Johan; Hakulinen, Nina; Andberg, Martina; Koivula, Anu; Rouvinen, Juha. 2021. Three-dimensional structure of xylonolactonase from Caulobacter crescentus: A mononuclear iron enzyme of the 6-bladed ß-propeller hydrolase family Protein science 2022; 31 2: 371-383. 2021
• Voutilainen, Sanni; Heinonen, Markus; Andberg, Martina; Jokinen, Emmi; Maaheimo, Hannu; Pääkkönen, Johan; Hakulinen, Nina; Rouvinen, Juha; Lähdesmäki, Harri; Kaski, Samuel; Rousu, Juho; Penttilä, Merja; Koivula, Anu. 2020. Substrate specificity of 2-deoxy-D-ribose 5-phosphate aldolase (DERA) assessed by different protein engineering and machine learning methods Applied microbiology and biotechnology 104: 10515-10529. 2020
• Haka, J; Niemi, MH; Mattila, P; Jänis, J; Takkinen, K; Rouvinen, J. 2019. Development of hypoallergenic variants of the major horse allergen Equ c 1 for immunotherapy by rational structure based engineering Scientific reports 9: 20148. 2019
• Kiema, TR; Thapa, CJ; Laitaoja, M; Schmitz, W; Maksimainen, MM; Fukao, T; Rouvinen, J; Jänis, J; Wierenga, RK. 2019. The peroxisomal zebrafish SCP2-thiolase (type-1) is a weak transient dimer as revealed by crystal structures and native mass spectrometry Biochemical journal 476 2: 307-332. 2019
• Takkinen, Kristiina; Rouvinen, Juha. 2019. Allergeenien ja IgE-vasta-aineiden rakennetutkimuksesta uuden sukupolven allergiarokotteiden kehitykseen Dosis 35 2: 176-185. 2019
• Thangaraj, Senthil K; James, Salman; Rouvinen, Juha; Jänis, Janne. 2019. Thermokinetic Analysis of Protein Subunit Exchange by Variable-Temperature Native Mass Spectrometry Biochemistry 58 50: 5025-5029. 2019
• Thangaraj, SK; Arola, H; Tullila, A; Nevanen, TK; Rouvinen, J; Jänis, J. 2019. Quantitation of Thyroid Hormone Binding to Anti-Thyroxine Antibody Fab Fragment by Native Mass Spectrometry ACS omega 4 20: 18718-18724. 2019
• Thangaraj, SK; Voutilainen, S; Andberg, M; Koivula, A; Jänis, J; Rouvinen, J. 2019. Bioconjugation with Aminoalkylhydrazine for Efficient Mass Spectrometry-Based Detection of Small Carbonyl Compounds ACS omega 4 8: 13447-13453. 2019
• Rahman, MM; Andberg, M; Koivula, A; Rouvinen, J; Hakulinen, N. 2018. The crystal structure of D-xylonate dehydratase reveals functional features of enzymes from the Ilv/ED dehydratase family. Scientific Reports 8 1: 865. 2018
• Penttinen, L; Rutanen, C; Saloheimo, M; Kruus, K; Rouvinen, J; Hakulinen, N. 2018. A new crystal form of Aspergillus oryzae catechol oxidase and evaluation of copper site structures in coupled binuclear copper enzymes. PLOS ONE 13 5: e0196691. 2018
• Penttinen, Leena; Rutanen, Chiara; Jänis, Janne; Rouvinen, Juha; Hakulinen, Nina. 2018. Unraveling substrate specificity and catalytic promiscuity of Aspergillus oryzae catechol oxidase CHEMBIOCHEM : A EUROPEAN JOURNAL OF CHEMICAL BIOLOGY 19 22: 2348-2352. 2018
• Abbott, Wade; Alber, Orly; Bayer, Ed; Berrin, Jean-Guy; Boraston, Alisdair; Brumer, Harry; Brzezinski, Ryszard; Clarke, Anthony; Cobucci-Ponzano, Beatrice; Cockburn, Darrell; Coutinho, Pedro; Czjzek, Mirjam; Dassa, Bareket; Davies, Gideon John; Eijsink, Vincent; Eklof, Jens; Felice, Alfons; Ficko-Blean, Elizabeth; Pincher, Geoff; Fontaine, Thierry et al. (Incl. Rouvinen, Juha). 2018. Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes. GLYCOBIOLOGY 28 1: 3-8. 2018
• Chu Yindi, Tu Tao, Penttinen Leena, Xue Xianli, Wang Xiaoyu, Yi Zhuolin, Gong Li, Rouvinen Juha, Luo Huiying, Hakulinen Nina, Yao Bin, Su Xiaoyun. 2017. Insights into the roles of non-catalytic residues in the active site of a GH10 xylanase with activity on cellulose. JOURNAL OF BIOLOGICAL CHEMISTRY 292 47: 19315-19327. 2017
• Rahman Mohammad Mubinur, Andberg Martina, Thangaraj Senthil Kumar, Parkkinen Tarja, Penttilä Merja, Jänis Janne, Koivula Anu, Rouvinen Juha, Hakulinen Nina. 2017. The Crystal Structure of a Bacterial L-Arabinonate Dehydratase Contains a [2Fe-2S] Cluster. ACS Chemical Biology 12 7: 1919-1927. 2017
• Banning A, Gülec C, Rouvinen J, Gray SJ, Tikkanen R. 2016. Identification of Small Molecule Compounds for Pharmacological Chaperone Therapy of Aspartylglucosaminuria. Scientific Reports 6: 37583. 2016
• Rahman Mohammad Mubinur, Andberg Martina, Koivula Aanu, Rouvinen Juha, Hakulinen Nina. 2016. Crystallization and X-ray diffraction analysis of an L-arabinonate dehydratase from Rhizobium leguminosarum bv. trifolii and a D-xylonate dehydratase from Caulobacter crescentus. Acta Crystallographica Section F 72 8: 604-608. 2016
• Taberman Helena, Parkkinen Tarja, Rouvinen Juha. 2016. Structural and functional features of the NAD(P) dependent Gfo/Idh/MocA protein family oxidoreductases. Protein Science 25 4: 778-786. 2016
• Taberman Helena, Andberg Martina, Koivula Anu, Hakulinen Nina, Penttilä Merja, Rouvinen Juha, Parkkinen Tarja. 2015. Structure and function of Caulobacter crescentus aldose-aldose oxidoreductase. BIOCHEMICAL JOURNAL 472 3: 297-307. 2015
• Niemi Merja H, Rytkönen-Nissinen Marja, Miettinen Ilja, Jänis Janne, Virtanen Tuomas, Rouvinen Juha. 2015. Dimerization of lipocalin allergens. Scientific Reports 5: 13841. 2015
• Hakulinen Nina, Rouvinen Juha. 2015. Three-dimensional structures of laccases. CELLULAR AND MOLECULAR LIFE SCIENCES: CMLS 72 5: 857-868. 2015
• Anbarasan Sasikala, Timoharju Tommi, Barthomeuf Janice, Pastinen Ossi, Rouvinen Juha, Leisola Matti, Turunen Ossi. 2015. Effect of active site mutation on pH activity and transglycosylation of Sulfolobus acidocaldarius ß-glycosidase. Journal of Molecular Catalysis B: Enzymatic 118: 62-69. 2015
• Vaarala O, Vuorela A, Partinen M, Baumann M, Freitag TL, Meri S, Saavalainen P, Jauhiainen M, Soliymani R, Kirjavainen T, Olsen P, Saarenpää-Heikkilä O, Rouvinen J, Roivainen M, Nohynek H, Jokinen J, Julkunen I, Kilpi T. 2014. Antigenic Differences between AS03 Adjuvanted Influenza A (H1N1) Pandemic Vaccines: Implications for Pandemrix-Associated Narcolepsy Risk. PLOS ONE [ONLINE] 9: e114361. 2014
• Niemi Merja H, Rytkönen-Nissinen Marja, Jänis Janne, Virtanen Tuomas, Rouvinen Juha. 2014. Structural aspects of dog allergies: The crystal structure of a dog dander allergen Can f 4. MOLECULAR IMMUNOLOGY 61 1: 7-15. 2014
• Taberman H, Andberg A, Parkkinen T, Jänis J, Penttilä M, Hakulinen N, Koivula A, Rouvinen J. 2014. Structure and function of a decarboxylating Agrobacterium tumefaciens keto-deoxy-D-galactarate dehydratase. BIOCHEMISTRY 53 51: 8052-8060. 2014
• Taberman H, Andberg M, Parkkinen Y, Richard P, Hakulinen N, Koivula A, Rouvinen J. 2014. Purification, crystallization and preliminary X-ray diffraction analysis of a novel keto-deoxy-D-galactarate (KDG) dehydratase from Agrobacterium tumefaciens. Acta Crystallographica Section F 70: 49-52. 2014
• Hakulinen N, Gasparetti C, Kaljunen H, Kruus K, Rouvinen J. 2013. The crystal structure of an extracellular catechol oxidase from the ascomycete fungus Aspergillus oryzae. Journal of biological inorganic chemistry 18: 917-929. 2013
• Maksimainen MM, Lampio A, Mertanen M, Turunen, O, Rouvinen J. 2013. The crystal structure of acidic beta-galactosidase from Aspergillus oryzae. International Journal of Biological Macromolecules 60: 109-115. 2013
• Maksimainen M, Paavilainen S, Hakulinen N, Rouvinen J. 2012. Structural analysis, enzymatic characterization, and catalytic mechanisms of beta-galactosidase from Bacillus circulans sp alkalophilus. FEBS JOURNAL 279: 1788-1798. 2012
• Laura Vepsäläinen, Katja Palmunen, Sinikka Uotila, Kalevi Visuri, Juha Rouvinen, and Johanna M. Kallio. 2011. Polysaccharides as Precipitants in Protein Crystallization for X-Ray Diffraction Studies CRYSTAL CROWTH & DESIGN 11: 1152-1158. 2011
• Parkkinen T, Boer H, Jänis J, Andberg M, Penttilä M, Koivula A & Rouvinen J. 2011. Crystal structure of uronate dehydrogenase from Agrobacterium tumefaciens JOURNAL OF BIOLOGICAL CHEMISTRY 286: 27294-27300. 2011
• Maksimainen M, Hakulinen N, Kallio JM, Timoharju T, Turunen O & Rouvinen J.. 2011. Crystal structures of Trichoderma reesei β-galactosidase reveal conformational changes in the active site JOURNAL OF STRUCTURAL BIOLOGY 174: 156-163. 2011
• Niemi MH, Takkinen K, Amundsen L, Söderlund H, Rouvinen J & Höyhtyä M. 2011. The testosterone binding mechanism of an antibody derived from a naïve human scFv library Journal of Molecular Recognition 24: 209-219. 2011
• Kallio JP, Gasparetti C, Andberg M, Boer H, Koivula A, Kruus K, Rouvinen J & Hakulinen N. 2011. Crystal structure of an ascomycete fungal laccase from Thielavia arenaria: Common features of asco-laccases FEBS JOURNAL 278: 2283-2295. 2011
• Kallio JM & Rouvinen J. 2011. Amphiphilic nanotubes in the crystal structure of a biosurfactant protein hydrophobin HFBII CHEMICAL COMMUNICATIONS 47: 9843-9845. 2011
• Kaljunen H, Gasparetti C, Kruus K, Rouvinen J & Hakulinen N. 2011. Crystallization and preliminary X-ray analysis of Aspergillus oryzae catechol oxidase Acta Crystallographica Section F 67: 672-674. 2011
• Kallio JP, Rouvinen J, Kruus K & Hakulinen N. 2011. Probing the dioxygen route in Melanocarpus albomyces laccase with pressurised xenon-gas BIOCHEMISTRY 50: 4396-4398. 2011
• Juha Rouvinen, Janne Jänis, Marja-Leena Laukkanen, Sirpa Jylhä, Merja Niemi, Tero Päivinen, Soili Mäkinen-Kiljunen, Tari Haahtela, Hans Söderlund, Kristiina Takkinen. 2010. Transient Dimers of Allergens. PLOS ONE [ONLINE] 5 2: e9037. 2010
• Merja H. Niemi, Laura Turunen, Timo Pulli, Tarja K. Nevanen, Matti Höyhtyä, Hans Söderlund, Juha Rouvinen, Kristiina Takkinen. 2010. A Structural Insight into the Molecular Recognition of a (-) -Delta 9-Tetrahydrocannabinol and the Development of a Sensitive, One-Step, Homogeneous Immunocomplex-Based Assay for Its Detection. JOURNAL OF MOLECULAR BIOLOGY 400 4: 803-814. 2010
• Andberg, M., Hakulinen, N., Auer, S., Saloheimo, M., Koivula, A., Rouvinen, J. and Kruus, K.. 2009. Essential role of the C-terminus in Melanocarpus albomyces laccase for enzyme production, catalytic properties and structure. FEBS Journal 276: 6285-6300. 2009
• Kallio JM, Hakulinen N, Kallio JP, Niemi MH, Kärkkäinen S & Rouvinen J. 2009. The contribution of polystyrene nanospheres towards the crystallization of proteins. PLOS ONE [ONLINE] 4 1: e4198. 2009
• Kallio, J. P., Auer, S., Jänis, J., Andberg, M., Kruus, K., Rouvinen, J., Koivula, A., and Hakulinen, N. 2009. Structure-function studies of a Melanocarpus albomyces laccase suggest a pathway for oxidation of phenolic compounds J. Mol. Biol. 392: 895-909. 2009
• Kallio, J. P., Jänis, J., Nyyssölä, A., Hakulinen, N., and Rouvinen, J.. 2009. Preliminary X-ray analysis of twinned crystals of sarcosine dimethylglycine methyltransferase from Halorhodospira halochoris. Acta Cryst. F65: 805-808. 2009
• Maksimainen M, Timoharju T, Kallio JM, Hakulinen N, Turunen O & Rouvinen J. 2009. Crystallization and preliminary diffractoin analysis of a b-galactosidase from Trichoderma reesei. Acta Cryst. F65: 767-769. 2009
• S. Saarelainen, M. Rytkönen-Nissinen, J. Rouvinen, A. Taivainen, S. Auriola, A. Kauppinen, T. Kinnunen and T. Virtanen. 2008. Animal-derived lipocalin allergens exhibit immunoglobulin E cross-reactivity. Clinical and Experimental Allergy 38: 374-381. 2008
• Tarja Parkkinen, Anu Koivula, Jari Vehmaanperä and Juha Rouvinen. 2008. Crystal structures of Melanocarpus albomyces cellobiohydrolase Cel7B in complex with cello-oligomers show high flexibility in the substrate binding. Protein Science 17: 1383-1394. 2008
• Jänis J, Rouvinen J, Vainiotalo P, Turunen O & Shnyrov VL. 2008. Irreversible thermal denaturation of Trichoderma reesei endo-1,4-b- xylanase II and its three disulfide mutants characterized by differential scanning calorimetry. International Journal of Biological Macromolecules 42: 75-80. 2008
• N. Hakulinen, M. Andberg, J. Kallio, A. Koivula, K. Kruus, J. Rouvinen. 2008. A near-atomic resolution structure of a Melanocapus albomyces laccase.. Journal of Structural Biology 162: 29-39. 2008
• Merja Niemi, Janne Jänis, Sirpa Jylhä, Johanna M. Kallio, Nina Hakulinen, Marja-Leena Laukkanen, Kristiina Takkinen and Juha Rouvinen. 2008. Characterization and crystallization of a recombinant IgE Fab fragment in complex with the bovine b- lactoglobulin allergen. Acta Crystallographica Section F F64: 25-28. 2008
• Janne Jänis, Salla Pasanen, Juha Rouvinen and Pirjo Vainiotalo. 2008. Characterization of the pH-dependent dissociation of a multimeric metalloprotein Streptomyces rubiginosus xylose isomerase by ESI FT-ICR mass spectrometry. Journal of Mass Spectrometry 43: 1376-1380. 2008
• J. Jänis;P. Pulkkinen;J. Rouvinen;P. Vainiotalo. 2007. Determination of steady-state kinetic parameters for a xylanase-catalyzed hydrolysis of neutral underivatized xylooligosaccharides by mass spectrometry. Anal. Biochem 365: 165-173. 2007
• Niemi M.;Jylhä S.;Laukkanen M-L.;Söderlund H.;Mäkinen-Kiljunen S.;Kallio J.;Hakulinen N.;Haahtela T.;Takkinen K.;Rouvinen J.. 2007. Molecular interactions between a recombinant IgE antibody and the b- lactoglobulin allergen. Structure 15: 1413-1421. 2007
• Parkkinen T.;Koivula A.;Vehmaanperä J.;Rouvinen J.. 2007. Preliminary X- ray analysis of cellobiohydrolase Cel7B from Melanocarpus albomyces. Acta Crystallographica Section F 63: 754-757. 2007
• Purmonen M.;Valjakka J.;Takkinen K.;Laitinen T.;Rouvinen J.. 2007. Molecular dynamics studies on thermostability of family 11 xylanases. Protein Engineering, Design & Selection 20: 551-559. 2007
• Voutilainen S.;Boer H.;Linder M.;Puranen T.;Rouvinen J.;Vehmaanperä J.;Koivula A.. 2007. Heterologous expression of Melanocarpus albomyces cellobiohydrolase Cel7B, and random mutagenesis to improve its thermostability Enzyme Microbiol Technology 41: 234-243. 2007
• Teerinen Tuija;Valjakka Jarkko;Rouvinen Juha;Takkinen Kristiina. 2006. Structure-based stability engineering of the mouse IgG1 fab fragment by modifying constant domains Journal of Molecular Biology 361: 687-697. 2006
• Parkkinen Tarja;Nevanen Tarja;Koivula Anu;Rouvinen Juha. 2006. Crystal Structures of an Enantioselective Fab-fragment in Free and Complex Forms. Journal of Molecular Biology 357: 471-480. 2006
• Hakulinen Nina;Kruus Kristiina;Koivula Anu;Rouvinen Juha. 2006. A crystallographic and spectroscopic study on the effect of X-ray radiation on the crystal structure of Melanocarpus albomyces laccase. Biochemical Biophysical Research Communications 350: 929-934. 2006
• Hakanpää J.;Szilvay G.;Kaljunen H.;Maksimainen M.;Linder M.;Rouvinen J.. 2006. Two crystal structures of Trichoderma reesei hydrophobin HFBI - a structure of a protein amphiphile with and without detergent interaction. Protein Science 15: 2129-2140. 2006
• Hakanpää J.;Linder M.;Popov A.;Schmidt A.;Rouvinen J.. 2006. Hydrophobin HFBII in detail - ultrahigh resolution structure of 0.75 Å. Acta Crystallographica Section D: Biological Crystallography D62: 356-367. 2006
• Jänis J;Hakanpää J;Hakulinen N;Ibatullin FM;Hoxha A;Derrick PJ;Rouvinen J;Vainiotalo P. 2005. Determination of Thiooxylo-oligosaccharide Binding to Family 11 Xylanases Using Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry and X-Ray Crystallography. FEBS Journal 272: 2317-2333. 2005
• Hakanpää J;Paananen A;Askolin S;Nakari-Setälä T;Parkkinen T;Penttilä M;Linder MB & Rouvinen J. 2004. Atomic Resolution Structure of the HFBII Hydrophobin, a Self-assembling Amphiphile J. Biol. Chem. 279: 534-539. 2004
• Hakanpää J;Parkkinen T;Hakulinen N;Linder M & Rouvinen J. 2004. Crystallization and preliminary X-ray characterization of Trichoderma reesei hydrophobin HFBII. Acta Cryst. D 60: 163-165. 2004
• Jari Palviainen;Juha Rouvinen and Raimo Silvennoinen. 2004. Analysis of microfibril angle of wood fibers using laser microscope polarimetry. Optical Engineering 43 1: 186-191. 2004
• Jokela M;Eskelinen A;Pospiech H;Rouvinen J;and Syväoja JE.. 2004. Characterization of the 3'exonuclease subunit DP1 of Methanococcus jannaschii replicative DNA polymerase D. Nucl Acids Res 32: 2430-2440. 2004
• Jänis J;Turunen O;Leisola M;Derrick PJ;Rouvinen J & Vainiotalo P. 2004. Characterization of Mutant Xylanases Using Fourier Transform Ion Cyclotron Resonance Mass Spectrometry: Stabilizing Contributions of Disulfide Bridges and N- Terminal Extensions. Biochemistry 43: 9556-9866. 2004
• Parkkinen T;Hakulinen N;Tenkanen M;Siika-aho M & Rouvinen J. 2004. Crystallization and preliminary X-ray analysis of a novel Trichoderma reesei xylanase IV belonging to glycoside hydrolase family 5. Acta Cryst. D 60: 542-544. 2004
• Saarela J;Oinonen C;Jalanko A;Rouvinen J & Peltonen L. 2004. Autoproteolytic activation of human aspartylglucosaminidase. Biochem. J. 378: 363-371. 2004
• Simonaho S-P;Tolonen Y;Rouvinen J & Silvennoinen R. 2004. Laser light scattering from wood samples soaked in water or in benzyl benzoate. Optik 114: 445-448. 2004
• Valjakka J & Rouvinen J. 2003. Crystal structure of 20k endoglucanase from Melanocarpus albomyces at 1.8 Å resolution.. Acta Cryst. D 59: 765-768. 2003
• Simo-Pekka Simonaho;Yrjö Tolonen;Juha Rouvinen and Raimo Silvennoinen. 2003. Laser light scattering from wood samples soaked in water and in bentzyl benzoate Optik 114 10: 445-448. 2003
• Hakulinen N;Turunen O;Jänis J;Leisola M & Rouvinen J:. 2003. Three-dimensional structures of thermophilic beta-1,4-xylanases from Chaetomium thermophilum and Nonomuraea flexuosa. Eur. J. Biochem. 270: 1399-1412. 2003
• Laitinen T;Rouvinen J & Peräkylä M. 2003. MMPBSA free energy analysis of endo1,4xylanase II (XynII)substrate complexes: binding of the reactive sugar in a skewboat and chair conformation.. Org. Biomolec. Chem. 1: 3535-3540. 2003
• Nousiainen Marjaana;Derrick Peter;Kaartinen;Mäenpää Pekka;Rouvinen Juha;Vainiotalo Pirjo. 2002. A Mass Spectrometric Study of Metal-Binding to Osteocalcin. Chemistry & Biology 9: 195-202. 2002
• Valjakka J.;Hemminki A.;Niemi S.;Söderlund H.;Takkinen K. & Rouvinen J.. 2002. Crystal structure of an in vitro affinity- and specificity-matured anti-testosterone Fab in complex with testosterone. Improved affinity results from small structural changes within tghe variable domains. J. Biol. Chem. 277: 44021-44027. 2002
• Valjakka J.;Takkinen K.;Teerinen T.;Söderlund H. & Rouvinen J.. 2002. Structural insights into steroid hormone binding. The Crystal structures of a recombinant anti-testosterone Fab fragment in free and testosterone-bound forms. J. Biol. Chem. 277: 4183-4190. 2002
• Jänis Janne;Rouvinen Juha;Leisola Matti;Turunen Ossi;Vainiotalo Pirjo. 2001. Thermostability of endo-1,4-beeta-Xylanase II from Trichoderma Reesei Studied by Electrospray Ionization Fourier-Transform Ion Cyclotron Resonance MS, Hydrogen/Deuterium-Exchange Reactions and Dynamic Light Scattering. Biochemical Journal 356: 453-460. 2001
• Rouvinen J;Virtanen T;Mäntyjärvi R. 2001. Searching for the determinants of allergenicity in proteins of the lipocalin family. J. Chromatogr. B. 756: 199-206. 2001
• Saarela J;Laine M;Oinonen C;von Schantz C;Jalanko A;Rouvinen J;Peltonen L. 2001. Molecular pathogenesis of a disease: structural consequences of aspartylglucosaminuria mutations. Human Mol. Genet. 10: 983-995. 2001
• Suihko Mona;Ahlgren Markku;Aulaskari Paula;Rouvinen Juha. 2001. Improved Synthesis and Characterization of 1,3,4,6-tetra-O-acetyl-2-(N-acetylacetamido)-2-deoxy-beta-D-glucopyranose Carbohydrate Research 334: 337-341. 2001
• Suihko M;Ahlgrén M;Aulaskari P;Rouvinen J. 2001. Improved synthesis and characterization of 1,3,4,6-tetra-O-acetyl-2-(N-acetylacetamido)-2-deoxy-b-D-glucopyranose Carbohydrate Res. 334: 337-341. 2001
• Turunen O;Etuaho K;Fenel F;Vehmaanperä J;Wu X;Rouvinen J;Leisola M. 2001. Combination of weakly stabilizing mutations with a disulfide bridge in the a-helix region of Trichoderma reesei endo-1,4-b-xylanase II increases the thermal stability through synergism. J. Biotechnol. 88: 37-46. 2001
• Muilu J;Törrönen A;Peräkylä M & Rouvinen J. 1998. Conformational changes and the catalytic cycle of endo-1,4-xylanase II from Trichoderma reesei: A molecular dynamics study. Proteins 31: 434-444. 1998
• Quack M;Szafranski K;Rouvinen J & Carlberg C. 1998. The role of the T-box for the function of the vitamin D receptor on different types of response elements. Nucl. Acids Res. 26: 5372-5378. 1998
• Koivula A.;Kinnari T;Harjunpää V;Ruohonen L;Teleman A;Drakenberg;T;Rouvinen J;Jones TA;Teeri TT. 1998. Tryptophan 272: an essential determinant of crystalline cellulose degradation by Trichoderma reesei cellobiohydrolase Cel6A. FEBS Lett. 429: 341-346. 1998
• Laitinen T;Rouvinen J;Peräkylä M. 1998. Ab initio quantum mechanical and density functional theory calculations of nucleophile- and nucleophile and acid-catalyzed opening of an epoxide ring: A model for the covalent binding of epoxyalkyl inhibitors to the active site of glycosidases. J. Org. Chem. 63: 8157-8162. 1998
• Hakulinen N;Tenkanen M;Rouvinen J. 1998. Crystallization and preliminary X-ray diffraction studies of the catalytic core of acetyl xylan esterase from Trichoderma reesei. Acta Cryst. D 54: 430-432. 1998
• Tikkanen R;Peltola M;Oinonen C;Rouvinen J;Peltonen L. 1997. Several cooperating binding sites mediate the interaction of a lysosomal enzyme with phosphotransferase EMBO J. 16: 6684-6693. 1997
• Törrönen A;Rouvinen J. 1997. Structural and functional properties of low molecular weight endo-1,4-b-xylanases Biotech. 57: 137-149. 1997
• Aulaskari;Paula;Ahlgren;Markku;Rouvinen;Juha;Vainiotalo;Pirjo;Pohjala;Esko;Vepsäläinen;Jouko. 1996. Preparation and Structure Determination of 1-Benzyl-, 1-Methyl- and 1H-5-[(2-Nitro-2-phenyl)ethenyl]imidazoles. Journal of Heterocyclic Chemistry 33: 1345-1354. 1996
• Havukainen R;Törrönen A;Laitinen T;Rouvinen J. 1996. Covalent Binding of Three Epoxyalkyl-Xylosides to the Active Site of Endo-1,4-Xylanase II from Trichoderma reesei. Biochemistry 35: 9617-9624. 1996
• Koivula A;Reinikainen T;Ruohonen L;Valkeajärvi A;Claeyssens M;Teleman O;Kleywegt GJ;Szardenings M;Rouvinen J;Jones TA;Teeri TT. 1996. The active site of Trichoderma reesei cellobiohydrolase II: the role of tyrosine 169. Protein Eng. 9: 691-699. 1996
• Peräkylä M;Rouvinen J. 1996. Ab Initio Quantum Mechanical Study on the Catalytic Mechanism of Aspartylglucosaminidase (AGA): A Serine Protease-like Mechanism with an N-terminal Threonine and Substrate-Assisted Catalysis. Chem. Eur. J. 2: 150-154. 1996
• Tikkanen R;Riikonen A;Oinonen C;Rouvinen J;Peltonen L. 1996. Functional analyses of active site residues of human lysosomal aspartylglucosaminidase: implications for catalytic mechanism and autocatalytic activation EMBO J. 15: 2954-2960. 1996
• Tikkanen R;Rouvinen J;Törrönen A;Kalkkinen N;Peltonen L. 1996. Large-Scale Purification and Preliminary X-ray Diffraction Studies of Human Aspartylglucosaminidase. Proteins 24: 253-258. 1996
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