European Network of Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Centers (EU FT-ICR MS)

Instruments available at UEF Chemistry

1) Bruker 12-T Solarix FT-ICR instrument

• Dynamically harmonized ICR cell (ParaCell), 12-T superconducting magnet
• Ionization: ESI, nanoESI, APPI, APCI, GC-APCI, DIP, DESI (Prosolia), microchip-ESI (Advion Triverse NanoMate)
• MS/MS: CID, SORI-CID, ECD/EDD, IRMPD, CASI/multi-CASI
• HPLC (Dionex Ultimate 3000 RPLC), GC (Bruker/Scion 456-GC with FID)
• Performance: m/z 90-15,0000; RMS mass error < 100 ppb; resolving power (FWHM) > 1,000,000 (broadband) @ m/z 400. Absorption mode acquisition.
• Software available: Bruker DataAnalysis, MetaboScape, TASQ, PetroOrg

2) Bruker timsTOF instrument

• First generation trapped ion mobility (TIMS) QTOF mass spectrometer
• Ionization: ESI, nanoESI, APPI, APCI, GC-APCI, DIP; MS/MS: low-energy CID
• HPLC (Dionex Ultimate 3000 RPLC), GC (Bruker/Scion 456-GC with FID)
• Performance: m/z 50-10,0000; RMS mass error < 1 ppm; resolving power (FWHM) > 70,000
• Software available in-house: Bruker DataAnalysis, MetaboScape, TASQ

3) Thermo Q Exactive Orbitrap UHMR instrument

• Ultra-high mass range (UHMR) Orbitrap mass spectrometer
• Ionization: ESI (HESI), off-line nanoESI; MS/MS: low- and high-energy CID (HCD)
• Performance: m/z 300-80,0000; RMS mass error < 1 ppm; resolving power (FWHM) > 250,000 @ m/z 400
• Software available in-house: Thermo Excalibur, Thermo Biopharma for Protein Analysis