
Human Brain Disease Modelling (Lehtonen lab)
We are also interested in building up a human organ-on-chip model for the blood-brain barrier (BBB) as the BBB and drugs’ ability to cross the BBB and reach the correct target is critical when designing drugs for neurological diseases. To this end, we utilize stem cell-derived human endothelial cells, astrocytes, and pericytes and combine them with organ-on-chip technology. The use of all human cells omits problems arising from cross-species differences, and the organ-on-chip technology mimics the physiological architecture of the BBB and fully control the cellular microenvironment. The chips are provided by Finnadvance – an Oulu-based startup company. Our goal is to use the BBB chips for testing novel drugs and biomolecules like extracellular vesicles to penetrate the BBB. We are also interested in using the model to uncover the molecular and transport mechanisms that regulate BBB permeability in physiological and pathological conditions.
Avainsanat
Ryhmän jäsenet - UEF
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Sarka Lehtonen Yliopistotutkija, Terveystieteiden tiedekunta, A. I. Virtanen -instituutti
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Jonna Niskanen Project Researcher, EDCMET, Terveystieteiden tiedekunta, Farmasian laitos
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Tuuli-Maria Sonninen Tohtoriopiskelija, Terveystieteiden tiedekunta, A. I. Virtanen -instituutti
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Lidiia Plotnikova Projektitutkija
Johtaja(t)
Tohtoriopiskelija
Tukihenkilöstö
UEFin ulkopuoliset ryhmän jäsenet
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Sanni Peltonen sannipel@student.uef.fi
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Sara Kälvälä sarsk@student.uef.fi
Yhteistyökumppanit - UEF
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AIVI kantasolukeskus Kantasolukeskus on A.I.Virtanen instituutissa t...
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Solu- ja kudoskuvantamisen yksikkö Kuvantamisydinpalvelumme tarjoaa käyttöön laitt...
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Martikainen lab Tutkimusryhmämme on kiinnostunut mitokondriotau...
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Neuroinflammation research group
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Skitsofrenian neurobiologia Tutkimusryhmän johtaja on professori Jari Tiiho...
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Biolääketieteellinen informatiikka We make use of large-scale biomedical data, suc...
Yhteistyökumppanit
Julkaisut
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A CX3CR1 Reporter hESC Line Facilitates Integrative Analysis of In-Vitro-Derived Microglia and Improved Microglia Identity upon Neuron-Glia Co-culture Grubman, Alexandra; Vandekolk, Teresa H; Schröder, Jan; Sun, Guizhi; Hatwell-Humble, Jessica; Chan, Jonathan; Oksanen, Minna; Lehtonen, Sarka; Hunt, Cameron; Koistinaho, Jari; Nilsson, Susan; Haynes, John; Pouton, Colin; Polo, Jose. 2020. A CX3CR1 Reporter hESC Line Facilitates Integrative Analysis of In-Vitro-Derived Microglia and Improved Microglia Identity upon Neuron-Glia Co-culture Stem cell reports 14: 1-15. 2020
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Proteostasis Disturbances and Inflammation in Neurodegenerative Diseases Sonninen, Tuuli-Maria; Goldsteins, Gundars; Laham-Karam, Nihay; Koistinaho, Jari; Lehtonen, Sarka. 2020. Proteostasis Disturbances and Inflammation in Neurodegenerative Diseases Cells 9 10: 2183. 2020
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Metabolic alterations in Parkinson's disease astrocytes Sonninen, Tuuli-Maria; Hämäläinen, Riikka H; Koskuvi, Marja; Oksanen, Minna; Shakirzyanova, Anastasia; Wojciechowski, Sara; Puttonen, Katja; Naumenko, Nikolay; Goldsteins, Gundars; Laham-Karam, Nihay; Lehtonen, Marko; Tavi, Pasi; Koistinaho, Jari; Lehtonen, Šárka. 2020. Metabolic alterations in Parkinson's disease astrocytes Scientific reports 10 1: 14474. 2020
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Altered Brain Endothelial Cell Phenotype from a Familial Alzheimer Mutation and Its Potential Implications for Amyloid Clearance and Drug Delivery Oikari, Lotta; Pandit, Rucha; Stewart, Romal; Cuní-López, Carla; Quek, Hazel; Sutharsan, Ratneswary; Rantanen, Laura; Oksanen, Minna; Lehtonen, Sarka; de Boer, Carmela Maria; Polo, Jose; Götz, Jürgen; Koistinaho, Jari; White, Anthony. 2020. Altered Brain Endothelial Cell Phenotype from a Familial Alzheimer Mutation and Its Potential Implications for Amyloid Clearance and Drug Delivery Stem cell reports 14: 1-16. 2020
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Metabolic and immune dysfunction of glia in neurodegenerative disorders: focus on iPSC models Rolova, Taisia; Lehtonen, Sárka; Goldsteins, Gundars; Kettunen, Pinja; Koistinaho, Jari. 2020. Metabolic and immune dysfunction of glia in neurodegenerative disorders: focus on iPSC models Stem cells First published: 03 December 2020: 1-14. 2020
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PSEN1ΔE9, APPswe, and APOE4 Confer Disparate Phenotypes in Human iPSC-Derived Microglia Konttinen, H; Cabral-da-Silva, MEC; Ohtonen, S; Wojciechowski, S; Shakirzyanova, A; Caligola, S; Giugno, R; Ishchenko, Y; Hernández, D; Fazaludeen, MF; Eamen, S; Budia, MG; Fagerlund, I; Scoyni, F; Korhonen, P; Huber, N; Haapasalo, A; Hewitt, AW; Vickers, J; Smith, GC; et al.. 2019. PSEN1ΔE9, APPswe, and APOE4 Confer Disparate Phenotypes in Human iPSC-Derived Microglia Stem cell reports 13 4: 669-683. 2019
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Dysfunction of Cellular Proteostasis in Parkinson's Disease Lehtonen, Š; Sonninen, TM; Wojciechowski, S; Goldsteins, G; Koistinaho, J. 2019. Dysfunction of Cellular Proteostasis in Parkinson's Disease Frontiers in neuroscience 13: 457. 2019
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Neurobiological roots of psychopathy Tiihonen, J; Koskuvi, M; Lähteenvuo, M; Virtanen, PLJ; Ojansuu, I; Vaurio, O; Gao, Y; Hyötyläinen, I; Puttonen, KA; Repo-Tiihonen, E; Paunio, T; Rautiainen, MR; Tyni, S; Koistinaho, J; Lehtonen, S. 2019. Neurobiological roots of psychopathy Molecular psychiatry [Epub ahead of print 27 Aug 2019]: 1-10. 2019
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Sex-specific transcriptional and proteomic signatures in schizophrenia Tiihonen, J; Koskuvi, M; Storvik, M; Hyötyläinen, I; Gao, Y; Puttonen, KA; Giniatullina, R; Poguzhelskaya, E; Ojansuu, I; Vaurio, O; Cannon, TD; Lönnqvist, J; Therman, S; Suvisaari, J; Kaprio, J; Cheng, L; Hill, AF; Lähteenvuo, M; Tohka, J; Giniatullin, R; et al.. 2019. Sex-specific transcriptional and proteomic signatures in schizophrenia Nature communications 10 1: 3933. 2019
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Nontargeted Metabolite Profiling of Induced Pluripotent Stem Cells (iPSCs) Derived Neural Cells: Insights Into Mechanisms of Brain Diseases Lehtonen Šárka, Lehtonen Marko, Koistinaho Jari. 2016. Nontargeted Metabolite Profiling of Induced Pluripotent Stem Cells (iPSCs) Derived Neural Cells: Insights Into Mechanisms of Brain Diseases. In: Tomizava Minoru (ed.) Pluripotent Stem Cells - From the Bench to the Clinic. Intech. s. 209-226. 2016