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Nina Hakulinen

Nina Hakulinen

University Lecturer

Faculty of Science, Forestry and Technology, Department of Chemistry

nina.hakulinen@uef.fi | +358 50 442 2318

0504422318
050 4422318
050 442 2318
+358 50 442 2318
+35850 442 2318
050 4422 318
+358 50 4422 318
+35850 4422 318
+358 50 4422318
+35850 4422318
+358504422318

Information Publications

PhD 2003, Post-doctoral Researcher 2003-2011, Adjunct Professor (Docent) “Structure-function studies of proteins” 2011, Academy of Finland Research Fellow 2012-2017, University Researcher 2017-

Research interests:
– Structural biology of industrial enzymes
– Enzymatic hydrolysis of lignocellulosic biomass
– Structure and function of metalloenzymes

Research groups

Bioorganic Chemistry 01.01.2010 -

Keywords

bio-organic chemistry biobased products bioeconomy biological chemistry biomass utilizing enzymes biorefining chemistry enzymes protein crystallography protein expression structural biology X-ray crystallography

Publications

50 items

Effectiveness of ruminal xylanase with an extra proline-rich C-terminus on lignocellulosic biomass degradation
Dong, Ruyue; Liao, Min; Liu, Xiaoqing; Penttinen, Leena; Hakulinen, Nina; Qin, Xing; Wang, Xiaolu; Huang, Huoqing; Luo, Huiying; Yao, Bin; Bai, Yingguo; Tu, Tao. 2023. Bioresource technology. 372:
Structural insight to elucidate the binding specificity of the anti-cortisol Fab fragment with glucocorticoids
Eronen, Veikko; Tullila, Antti; Iljin, Kristiina; Rouvinen, Juha; Nevanen, Tarja K; Hakulinen, Nina. 2023. Journal of structural biology. 215:
Boosting enzymatic degradation of cellulose using a fungal expansin: Structural insight into the pretreatment mechanism
Ding, Sunjia; Liu, Xiaoqing; Hakulinen, Nina; Taherzadeh, Mohammad J.; Wang, Yaru; Wang, Yuan; Qin, Xing; Wang, Xiaolu; Yao, Bin; Luo, Huiying; Tu, Tao. 2022. Bioresource technology. 358:
Patulin Detoxification by Recombinant Manganese Peroxidase from Moniliophthora roreri Expressed by Pichia pastoris
Wang, Shuai; Wang, Xiaolu; Penttinen, Leena; Luo, Huiying; Zhang, Yuhong; Liu, Bo; Yao, Bin; Hakulinen, Nina; Zhang, Wei; Su, Xiaoyun. 2022. Toxins. 14:
Structure and function of aldopentose catabolism enzymes involved in oxidative non-phosphorylative pathways
Ren, Yaxin; Eronen, Veikko; Blomster Andberg, Martina; Koivula, Anu; Hakulinen, Nina. 2022. Biotechnology for biofuels and bioproducts. 15: . 147
Crystallization of 3-hexulose-6-phosphate synthase
Delfi, Masoud; Mahdavian, Leila; Sattarifar, Mohammad; Hakulinen, Nina; Rouvinen, Juha. 2021. European journal of chemistry. 12: 299-303
Current state of and need for enzyme engineering of 2-deoxy-D-ribose 5-phosphate aldolases and its impact
Rouvinen, Juha; Andberg, Martina; Pääkkönen, Johan; Hakulinen, Nina; Koivula, Anu. 2021. Applied microbiology and biotechnology. 105: 6215-6228
Cysteine Engineering of an Endo-polygalacturonase from Talaromyces leycettanus JCM 12802 to Improve Its Thermostability
Wang, S; Meng, K; Su, X; Hakulinen, N; Wang, Y; Zhang, J; Luo, H; Yao, B; Huang, H; Tu, T. 2021. Journal of agricultural and food chemistry. 69: 6351-6359
Polysaccharide utilization loci-driven enzyme discovery reveals BD-FAE: a bifunctional feruloyl and acetyl xylan esterase active on complex natural xylans
Hameleers, Lisanne; Penttinen, Leena; Ikonen, Martina; Jaillot, Léa; Fauré, Régis; Terrapon, Nicolas; Deuss, Peter J.; Hakulinen, Nina; Master, Emma R.; Jurak, Edita. 2021. Biotechnology for biofuels. 14: 127
Structural Insights into the Mechanisms Underlying the Kinetic Stability of GH28 Endo-Polygalacturonase
Tu, Tao; Wang, Zhiyun; Luo, Yan; Li, Yeqing; Su, Xiaoyun; Wang, Yuan; Zhang, Jie; Rouvinen, Juha; Yao, Bin; Hakulinen, Nina; Luo, Huiying. 2021. Journal of agricultural and food chemistry. 69: 815-823
Three-dimensional structure of xylonolactonase from Caulobacter crescentus: A mononuclear iron enzyme of the 6-bladed ß-propeller hydrolase family
Pääkkönen, Johan; Hakulinen, Nina; Andberg, Martina; Koivula, Anu; Rouvinen, Juha. 2021. Protein science. 2022; 31: 371-383
Xylonolactonase from Caulobacter crescentus Is a Mononuclear Nonheme Iron Hydrolase
Pääkkönen, Johan; Penttinen, Leena; Andberg, Martina; Koivula, Anu; Hakulinen, Nina; Rouvinen, Juha; Jänis, Janne. 2021. Biochemistry. 60: 3046-3049
Improving the catalytic performance of Proteinase K from Parengyodontium album for use in feather degradation
Ren, Y; Luo, H; Huang, H; Hakulinen, N; Wang, Y; Wang, Y; Su, X; Bai, Y; Zhang, J; Yao, B; Wang, G; Tu, T. 2020. International journal of biological macromolecules. 154: 1586-1595
Substrate specificity of 2-deoxy-D-ribose 5-phosphate aldolase (DERA) assessed by different protein engineering and machine learning methods
Voutilainen, Sanni; Heinonen, Markus; Andberg, Martina; Jokinen, Emmi; Maaheimo, Hannu; Pääkkönen, Johan; Hakulinen, Nina; Rouvinen, Juha; Lähdesmäki, Harri; Kaski, Samuel; Rousu, Juho; Penttilä, Merja; Koivula, Anu. 2020. Applied microbiology and biotechnology. 104: 10515-10529
A new crystal form of Aspergillus oryzae catechol oxidase and evaluation of copper site structures in coupled binuclear copper enzymes
Penttinen, L; Rutanen, C; Saloheimo, M; Kruus, K; Rouvinen, J; Hakulinen, N. 2018. PLoS ONE. 13: e0196691
The crystal structure of D-xylonate dehydratase reveals functional features of enzymes from the Ilv/ED dehydratase family
Rahman, MM; Andberg, M; Koivula, A; Rouvinen, J; Hakulinen, N. 2018. Scientific reports. 8: 865
Unraveling substrate specificity and catalytic promiscuity of Aspergillus oryzae catechol oxidase
Penttinen, Leena; Rutanen, Chiara; Jänis, Janne; Rouvinen, Juha; Hakulinen, Nina. 2018. Chembiochem. 19: 2348-2352
Insights into the roles of non-catalytic residues in the active site of a GH10 xylanase with activity on cellulose
Chu Yindi, Tu Tao, Penttinen Leena, Xue Xianli, Wang Xiaoyu, Yi Zhuolin, Gong Li, Rouvinen Juha, Luo Huiying, Hakulinen Nina, Yao Bin, Su Xiaoyun. 2017. Journal of biological chemistry. 292: 19315-19327
The Crystal Structure of a Bacterial L-Arabinonate Dehydratase Contains a [2Fe-2S] Cluster
Rahman Mohammad Mubinur, Andberg Martina, Thangaraj Senthil Kumar, Parkkinen Tarja, Penttilä Merja, Jänis Janne, Koivula Anu, Rouvinen Juha, Hakulinen Nina. 2017. Acs chemical biology. 12: 1919-1927
Characterization and mutagenesis of two novel iron - sulphur cluster pentonate dehydratases
Andberg M, Aro-Kärkkäinen N, Carlson P, Oja M, Bozonnet S, Toivari M, Hakulinen N, O'Donohue M, Penttilä M, Koivula A. 2016. Applied microbiology and biotechnology. 100: 7549-7563
Crystallization and X-ray diffraction analysis of an L-arabinonate dehydratase from Rhizobium leguminosarum bv. trifolii and a D-xylonate dehydratase from Caulobacter crescentus
Rahman Mohammad Mubinur, Andberg Martina, Koivula Aanu, Rouvinen Juha, Hakulinen Nina. 2016. Acta crystallographica section F : structural biology and crystallization communications. 72: 604-608
Structure and function of Caulobacter crescentus aldose-aldose oxidoreductase
Taberman Helena, Andberg Martina, Koivula Anu, Hakulinen Nina, Penttilä Merja, Rouvinen Juha, Parkkinen Tarja. 2015. Biochemical journal. 472: 297-307
Three-dimensional structures of laccases
Hakulinen Nina, Rouvinen Juha. 2015. Cellular and molecular life sciences. 72: 857-868
Purification, crystallization and preliminary X-ray diffraction analysis of a novel keto-deoxy-D-galactarate (KDG) dehydratase from Agrobacterium tumefaciens
Taberman H, Andberg M, Parkkinen Y, Richard P, Hakulinen N, Koivula A, Rouvinen J. 2014. Acta crystallographica section F : structural biology and crystallization communications. 70: 49-52
Structure and function of a decarboxylating Agrobacterium tumefaciens keto-deoxy-D-galactarate dehydratase
Taberman H, Andberg A, Parkkinen T, Jänis J, Penttilä M, Hakulinen N, Koivula A, Rouvinen J. 2014. Biochemistry. 53: 8052-8060
The crystal structure of an extracellular catechol oxidase from the ascomycete fungus Aspergillus oryzae
Hakulinen N, Gasparetti C, Kaljunen H, Kruus K, Rouvinen J. 2013. Journal of biological inorganic chemistry. 18: 917-929
Structural analysis, enzymatic characterization, and catalytic mechanisms of beta-galactosidase from Bacillus circulans sp alkalophilus
Maksimainen M, Paavilainen S, Hakulinen N, Rouvinen J. 2012. Febs journal. 279: 1788-1798
Crystal structure of an ascomycete fungal laccase from Thielavia arenaria: Common features of asco-laccases
Kallio JP, Gasparetti C, Andberg M, Boer H, Koivula A, Kruus K, Rouvinen J & Hakulinen N. 2011. Febs journal. 278: 2283-2295
Crystal structures of Trichoderma reesei &#946 -galactosidase reveal conformational changes in the active site
Maksimainen M, Hakulinen N, Kallio JM, Timoharju T, Turunen O & Rouvinen J.. 2011. Journal of structural biology. 174: 156-163
Crystallization and preliminary X-ray analysis of Aspergillus oryzae catechol oxidase
Kaljunen H, Gasparetti C, Kruus K, Rouvinen J & Hakulinen N. 2011. Acta crystallographica section F : structural biology and crystallization communications. 67: 672-674
Linear birefringence magnitude of artificial self-assembled DNA crystals
Lehmuskero Anni, Hakulinen Nina, Lajunen Hanna, Zheng Jianping, Sha Ruojie, Bai Benfeng, Huang Lingling, Turunen Jari, Vahimaa Pasi, Seeman Nadrian C.. 2011. Optical materials express. 1: 936-942
Polarization effects of self-assembled DNA crystals
Lehmuskero Anni, Hakulinen Nina, Vahimaa Pasi, Nadrian C. Seeman. 2011.
Probing the dioxygen route in Melanocarpus albomyces laccase with pressurised xenon-gas
Kallio JP, Rouvinen J, Kruus K & Hakulinen N. 2011. Biochemistry. 50: 4396-4398
Crystallization and preliminary diffractoin analysis of a b-galactosidase from Trichoderma reesei
Maksimainen M, Timoharju T, Kallio JM, Hakulinen N, Turunen O & Rouvinen J. 2009. Acta Cryst.. F65: 767-769
Essential role of the C-terminus in Melanocarpus albomyces laccase for enzyme production, catalytic properties and structure
Andberg, M., Hakulinen, N., Auer, S., Saloheimo, M., Koivula, A., Rouvinen, J. and Kruus, K.. 2009. FEBS Journal. 276: 6285-6300
Preliminary X-ray analysis of twinned crystals of sarcosine dimethylglycine methyltransferase from Halorhodospira halochoris
Kallio, J. P., Jänis, J., Nyyssölä, A., Hakulinen, N., and Rouvinen, J.. 2009. Acta Cryst.. F65: 805-808
Structure-function studies of a Melanocarpus albomyces laccase suggest a pathway for oxidation of phenolic compounds
Kallio, J. P., Auer, S., Jänis, J., Andberg, M., Kruus, K., Rouvinen, J., Koivula, A., and Hakulinen, N. 2009. J. Mol. Biol.. 392: 895-909
The contribution of polystyrene nanospheres towards the crystallization of proteins
Kallio JM, Hakulinen N, Kallio JP, Niemi MH, Kärkkäinen S & Rouvinen J. 2009. PLoS ONE. 4: e4198
A near-atomic resolution structure of a Melanocapus albomyces laccase.
N. Hakulinen, M. Andberg, J. Kallio, A. Koivula, K. Kruus, J. Rouvinen. 2008. Journal of Structural Biology. 162: 29-39
Characterization and crystallization of a recombinant IgE Fab fragment in complex with the bovine b- lactoglobulin allergen
Merja Niemi, Janne Jänis, Sirpa Jylhä, Johanna M. Kallio, Nina Hakulinen, Marja-Leena Laukkanen, Kristiina Takkinen and Juha Rouvinen. 2008. Acta crystallographica section F : structural biology and crystallization communications. F64: 25-28
Molecular interactions between a recombinant IgE antibody and the b- lactoglobulin allergen
Niemi M.;Jylhä S.;Laukkanen M-L.;Söderlund H.;Mäkinen-Kiljunen S.;Kallio J.;Hakulinen N.;Haahtela T.;Takkinen K.;Rouvinen J.. 2007. Structure. 15: 1413-1421
A crystallographic and spectroscopic study on the effect of X-ray radiation on the crystal structure of Melanocarpus albomyces laccase
Hakulinen Nina;Kruus Kristiina;Koivula Anu;Rouvinen Juha. 2006. Biochemical Biophysical Research Communications. 350: 929-934
Determination of Thiooxylo-oligosaccharide Binding to Family 11 Xylanases Using Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry and X-Ray Crystallography
Jänis J;Hakanpää J;Hakulinen N;Ibatullin FM;Hoxha A;Derrick PJ;Rouvinen J;Vainiotalo P. 2005. FEBS Journal. 272: 2317-2333
Crystallization and preliminary X-ray analysis of a novel Trichoderma reesei xylanase IV belonging to glycoside hydrolase family 5
Parkkinen T;Hakulinen N;Tenkanen M;Siika-aho M & Rouvinen J. 2004. Acta Cryst. D. 60: 542-544
Crystallization and preliminary X-ray characterization of Trichoderma reesei hydrophobin HFBII
Hakanpää J;Parkkinen T;Hakulinen N;Linder M & Rouvinen J. 2004. Acta Cryst. D. 60: 163-165
Three-dimensional structures of thermophilic beta-1,4-xylanases from Chaetomium thermophilum and Nonomuraea flexuosa
Hakulinen N;Turunen O;Jänis J;Leisola M & Rouvinen J:. 2003. Eur. J. Biochem.. 270: 1399-1412
Crystal structure of a laccase from Melanocarpus albomyces with an intact trinuclear copper site
Hakulinen N;Kiiskinen L-L;Kruus K;Saloheimo M;Paananen A;Koivula A;Rouvinen J. 2002. Nature structural biology. 9: 601-605
The crystal structure of beta-glucosidase from Bacillus circulans sp. alkalophilus: ability to form polymeric assemblies
Hakulinen N;Paavilainen S;Korpela T;Rouvinen J. 2000. Journal of Structural Biology. 129: 69-79
Three-dimensional structure of the catalytic core of acetylxylan esterase from Trichoderma reesei: insights into the deacetylation mechanism
Hakulinen N;Tenkanen M;Rouvinen J. 2000. Journal of Structural Biology. 132: 180-190
Crystallization and preliminary X-ray diffraction studies of the catalytic core of acetyl xylan esterase from Trichoderma reesei
Hakulinen N;Tenkanen M;Rouvinen J. 1998. Acta Cryst. D. 54: 430-432

University of Eastern Finland Joensuu, Kuopio © University of Eastern Finland

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Nina Hakulinen

Research groups

Keywords

Effectiveness of ruminal xylanase with an extra proline-rich C-terminus on lignocellulosic biomass degradation

Structural insight to elucidate the binding specificity of the anti-cortisol Fab fragment with glucocorticoids

Boosting enzymatic degradation of cellulose using a fungal expansin: Structural insight into the pretreatment mechanism

Patulin Detoxification by Recombinant Manganese Peroxidase from Moniliophthora roreri Expressed by Pichia pastoris

Structure and function of aldopentose catabolism enzymes involved in oxidative non-phosphorylative pathways

Crystallization of 3-hexulose-6-phosphate synthase

Current state of and need for enzyme engineering of 2-deoxy-D-ribose 5-phosphate aldolases and its impact

Cysteine Engineering of an Endo-polygalacturonase from Talaromyces leycettanus JCM 12802 to Improve Its Thermostability

Polysaccharide utilization loci-driven enzyme discovery reveals BD-FAE: a bifunctional feruloyl and acetyl xylan esterase active on complex natural xylans

Structural Insights into the Mechanisms Underlying the Kinetic Stability of GH28 Endo-Polygalacturonase

Three-dimensional structure of xylonolactonase from Caulobacter crescentus: A mononuclear iron enzyme of the 6-bladed ß-propeller hydrolase family

Xylonolactonase from Caulobacter crescentus Is a Mononuclear Nonheme Iron Hydrolase

Improving the catalytic performance of Proteinase K from Parengyodontium album for use in feather degradation

Substrate specificity of 2-deoxy-D-ribose 5-phosphate aldolase (DERA) assessed by different protein engineering and machine learning methods

A new crystal form of Aspergillus oryzae catechol oxidase and evaluation of copper site structures in coupled binuclear copper enzymes

The crystal structure of D-xylonate dehydratase reveals functional features of enzymes from the Ilv/ED dehydratase family

Unraveling substrate specificity and catalytic promiscuity of Aspergillus oryzae catechol oxidase

Insights into the roles of non-catalytic residues in the active site of a GH10 xylanase with activity on cellulose

The Crystal Structure of a Bacterial L-Arabinonate Dehydratase Contains a [2Fe-2S] Cluster

Characterization and mutagenesis of two novel iron - sulphur cluster pentonate dehydratases

Crystallization and X-ray diffraction analysis of an L-arabinonate dehydratase from Rhizobium leguminosarum bv. trifolii and a D-xylonate dehydratase from Caulobacter crescentus

Structure and function of Caulobacter crescentus aldose-aldose oxidoreductase

Three-dimensional structures of laccases

Purification, crystallization and preliminary X-ray diffraction analysis of a novel keto-deoxy-D-galactarate (KDG) dehydratase from Agrobacterium tumefaciens

Structure and function of a decarboxylating Agrobacterium tumefaciens keto-deoxy-D-galactarate dehydratase

The crystal structure of an extracellular catechol oxidase from the ascomycete fungus Aspergillus oryzae

Structural analysis, enzymatic characterization, and catalytic mechanisms of beta-galactosidase from Bacillus circulans sp alkalophilus

Crystal structure of an ascomycete fungal laccase from Thielavia arenaria: Common features of asco-laccases

Crystal structures of Trichoderma reesei &#946 -galactosidase reveal conformational changes in the active site

Crystallization and preliminary X-ray analysis of Aspergillus oryzae catechol oxidase

Linear birefringence magnitude of artificial self-assembled DNA crystals

Polarization effects of self-assembled DNA crystals

Probing the dioxygen route in Melanocarpus albomyces laccase with pressurised xenon-gas

Crystallization and preliminary diffractoin analysis of a b-galactosidase from Trichoderma reesei

Essential role of the C-terminus in Melanocarpus albomyces laccase for enzyme production, catalytic properties and structure

Preliminary X-ray analysis of twinned crystals of sarcosine dimethylglycine methyltransferase from Halorhodospira halochoris

Structure-function studies of a Melanocarpus albomyces laccase suggest a pathway for oxidation of phenolic compounds

The contribution of polystyrene nanospheres towards the crystallization of proteins

A near-atomic resolution structure of a Melanocapus albomyces laccase.

Characterization and crystallization of a recombinant IgE Fab fragment in complex with the bovine b- lactoglobulin allergen

Molecular interactions between a recombinant IgE antibody and the b- lactoglobulin allergen

A crystallographic and spectroscopic study on the effect of X-ray radiation on the crystal structure of Melanocarpus albomyces laccase

Determination of Thiooxylo-oligosaccharide Binding to Family 11 Xylanases Using Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry and X-Ray Crystallography

Crystallization and preliminary X-ray analysis of a novel Trichoderma reesei xylanase IV belonging to glycoside hydrolase family 5

Crystallization and preliminary X-ray characterization of Trichoderma reesei hydrophobin HFBII

Three-dimensional structures of thermophilic beta-1,4-xylanases from Chaetomium thermophilum and Nonomuraea flexuosa

Crystal structure of a laccase from Melanocarpus albomyces with an intact trinuclear copper site

The crystal structure of beta-glucosidase from Bacillus circulans sp. alkalophilus: ability to form polymeric assemblies

Three-dimensional structure of the catalytic core of acetylxylan esterase from Trichoderma reesei: insights into the deacetylation mechanism

Crystallization and preliminary X-ray diffraction studies of the catalytic core of acetyl xylan esterase from Trichoderma reesei