



Tumor-stroma interaction - Pasonen-Seppänen group
01.09.2019 -
Research group
Tumor microenvironment (TME) has an active contribution to tumor development and cancer progression. This extremely complex system is composed of multiple cell types, such as fibroblasts and infiltrating immune cells, and structural extracellular matrix (ECM), which is mainly composed of hyaluronan in many cancers. Tumor and stromal cells are in constant interaction with each other and together they remodel TME favourable for cancer spreading.
The focus of our research is on tumor microenvironment (TME). Our overall goal is to elucidate how stromal cells, like fibroblasts and immune cells, and extracellular matrix molecules like hyaluronan affect melanoma progression. We are interested in tumor immunology, the role of macrophages and FoxP3+ regulatory T-cells in the formation of immunosuppressive TME in melanoma. The role of immune cells in tumor dissemination is well established yet the mechanisms that recruit the immune cells and regulate their function in the TME are poorly understood at the moment and are the targets of our research. In addition, we are seeking for new prognostic and diagnostic biomarkers for melanoma.
Group description
Homepage of the groupKeywords
3D -cell culture models
cancer
confocal microscopy
ekstracellular matrix
extracellular vesicle
hyaluronan
immune signature
immunohistochemistry
macrophage
melanoma
pro-tumor inflammation
RNAseq
tumor microenvironment
tumor-stroma interaction
Time period
01.09.2019 -
Group members - UEF
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Sanna Pasonen-Seppänen University Lecturer , School of Medicine, Biomedicine
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Piia Takabe University Teacher , School of Medicine, Biomedicine
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Kirsi Kainulainen Early Stage Researcher , School of Medicine, Biomedicine
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Eija Rahunen Senior Laboratory Technician , School of Medicine, Biomedicine
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Taija Hukkanen Bioanalyst , School of Medicine, Biomedicine
Others
Other group members
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Mari Valkonen Mari.Valkonen@kuh.fi
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Satu Salmi Satu.Salmi@uef.fi
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Otto Jokelainen Otto.Jokelainen@kuh.fi
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Kaisa Mäki-Mantila kaim@student.uef.fi
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Lasse Hämäläinen Lasse.Hamalainen@uef.fi
Cooperation partners
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Kuopio University Hospital / FICAN East
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UEF
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UEF / Kuopio University Hospital
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UEF
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Münster University Hospital, Münster, Germany
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Lerner Research Institute, Cleveland Clinic, Cleveland, USA
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UEF
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UEF
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University of Turku
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UEF, Faculty of Health Sciences
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Kuopio University Hospital
Publications
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The calcium-activated chloride channel-associated protein rCLCA2 is expressed throughout rat epidermis, facilitates apoptosis and is downmodulated by UVB Hämäläinen, L; Bart, G; Takabe, P; Rauhala, L; Deen, A; Pasonen-Seppänen, S; Kärkkäinen, E; Kärnä, R; Kumlin, T; Tammi, M; I; Tammi, RH. 2021. The calcium-activated chloride channel-associated protein rCLCA2 is expressed throughout rat epidermis, facilitates apoptosis and is downmodulated by UVB Histochemistry and cell biology 5 1: 605-615. 2021
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The role of FoxP3+ regulatory T cells and IDO+ immune and tumor cells in malignant melanoma - an immunohistochemical study Salmi, Satu; Lin, Anton; Hirschovits-Gerz, Benjamin; Valkonen, Mari; Aaltonen, Niina; Sironen, Reijo; Siiskonen, Hanna; Pasonen-Seppänen, Sanna. 2021. The role of FoxP3+ regulatory T cells and IDO+ immune and tumor cells in malignant melanoma - an immunohistochemical study Bmc cancer 21 1: 641. 2021
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Cellular hyaluronan is associated with a poor prognosis in renal cell carcinoma Jokelainen, Otto; Pasonen-Seppänen, Sanna; Tammi, Markku; Mannermaa, Arto; Aaltomaa, Sirpa; Sironen, Reijo; Nykopp, Timo K. 2020. Cellular hyaluronan is associated with a poor prognosis in renal cell carcinoma Urologic oncology: seminars and original investigations 38 8: 686.e11-686.e22. 2020
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Activated hyaluronan metabolism in the tumor matrix - Causes and consequences Tammi, Markku I; Oikari, Sanna; Pasonen-Seppänen, Sanna; Rilla, Kirsi; Auvinen, Päivi; Tammi, Raija H. 2019. Activated hyaluronan metabolism in the tumor matrix - Causes and consequences Matrix biology 78-79: 147-164. 2019
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HAS3-induced extracellular vesicles from melanoma cells stimulate IHH mediated c-Myc upregulation via the hedgehog signaling pathway in target cells Arasu, UT; Deen, AJ; Pasonen-Seppänen, S; Heikkinen, S; Lalowski, M; Kärnä, R; Härkönen, K; Mäkinen, P; Lázaro-Ibáñez, E; Siljander, PR; Oikari, S; Levonen, AL; Rilla, K. 2019. HAS3-induced extracellular vesicles from melanoma cells stimulate IHH mediated c-Myc upregulation via the hedgehog signaling pathway in target cells Cellular and molecular life sciences [Published: 09 December 2019]: 1-23. 2019
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Melanocyte hyaluronan coat fragmentation enhances the UVB-induced TLR-4 receptor signaling and expression of proinflammatory mediators IL-6, IL-8, CXCL-1 and CXCL-10 via NF-κB activation Takabe, P; Kärnä, R; Rauhala, L; Tammi, M; Tammi, R; Pasonen-Seppänen, S. 2019. Melanocyte hyaluronan coat fragmentation enhances the UVB-induced TLR-4 receptor signaling and expression of proinflammatory mediators IL-6, IL-8, CXCL-1 and CXCL-10 via NF-κB activation Journal of investigative dermatology 139 9: 1993-2003.e4. 2019
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Elevated expression of hyaluronan synthase 2 associates with decreased survival in diffusely infiltrating astrocytomas Valkonen, Mari; Haapasalo, Hannu; Rilla, Kirsi; Tyynelä-Korhonen, Kristiina; Soini, Ylermi; Pasonen-Seppänen, Sanna. 2018. Elevated expression of hyaluronan synthase 2 associates with decreased survival in diffusely infiltrating astrocytomas Bmc cancer 18 1: 664. 2018
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Extracellular ATP activates hyaluronan synthase 2 (HAS2) in epidermal keratinocytes via P2Y2, Ca2+-signaling, and MAPK pathways Rauhala, L; Jokela, T; Kärnä, R; Bart, G; Takabe, P: Oikari, S; Tammi, MI; Pasonen-Seppänen, S; Tammi, RH. 2018. Extracellular ATP activates hyaluronan synthase 2 (HAS2) in epidermal keratinocytes via P2Y2, Ca2+-signaling, and MAPK pathways Biochemical journal 475 10: 1755-1772. 2018
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Hyaluronan metabolism enhanced during epidermal differentiation is suppressed by vitamin C Hämäläinen, L; Kärkkäinen, E; Takabe, P; Rauhala, L; Bart, G; Kärnä, R; Pasonen-Seppänen, S; Oikari, S; Tammi, MI; Tammi, RH. 2018. Hyaluronan metabolism enhanced during epidermal differentiation is suppressed by vitamin C British journal of dermatology 179 3: 651-661. 2018
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The number and localization of CD68+ and CD163+ macrophages in different stages of cutaneous melanoma Salmi, Satu; Siiskonen, Hanna; Sironen, Reijo; Tyynelä-Korhonen, Kristiina; Hirschovits-Gerz, Benjamin; Valkonen, Mari; Auvinen, Päivi; Pasonen-Seppänen, Sanna. 2018. The number and localization of CD68+ and CD163+ macrophages in different stages of cutaneous melanoma Melanoma research 29 3: 237-247. 2018